| 出品公司: | Promega |
|---|---|
| 载体名称: | pBiT2.1-N[TK/SmBiT] |
| 载体又名: | pBiT2.1-N[TK/SmBiT]载体，pBiT2.1-N[TK/SmBiT]质粒，pBiT2.1-N[TK/SmBiT]载体质粒 |
| 英文名称: | pBiT2.1-N[TK/SmBiT], pBiT2.1-N[TK/SmBiT] Vector, pBiT2.1-N[TK/SmBiT] Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | HSV TK |
| 载体大小: | 3.4 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | N20242175 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pBiT2.1-N[TK/SmBiT]
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pBiT2.1-N[TK/SmBiT]载体质粒基本信息
pBiT2.1-N[TK/SmBiT]质粒图谱载体图谱和pBiT2.1-N[TK/SmBiT]载体序列质粒序列多克隆位点信息
![pBiT2.1-N[TK/SmBiT]载体图谱](/uploads/载体图谱/pBiT2.1-N[TK-SmBiT]载体图谱.png)
![pBiT2.1-N[TK/SmBiT]多克隆位点](/uploads/载体图谱/pBiT2.1-N[TK/SmBiT]-MCS.png "pBiT2.1-N[TK/SmBiT]载体多克隆位点")
pBiT2.1-N[TK/SmBiT]质粒载体简介
pBiT2.1-N[TK/SmBiT]质粒序列载体序列
Snapgene载体序列下载: pBiT2.1-N[TK/LgBiT]序列 .gb格式载体序列下载: pBiT1.1-N[TK/LgBiT]载体序列
LOCUS pBiT2.1-N[TK-SmBiT] 3417 bp DNA circular SYN 19-JUL-2026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3417)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1135209600|
COMMENT VNTDBDATE|1135209600|
COMMENT VNTNAME|pBiT2.1-N[TK-SmBiT]|
COMMENT VNTAUTHORNAME|DotmaticsRD|
FEATURES Location/Qualifiers
promoter 28..779
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
regulatory 809..818
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 971..1092
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1511..2099)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(2299..3090,3091..3159))
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
polyA_signal 3264..3312
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3326..3417
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga gcggtggagc
901 tcaggggaat tcagtctaag ctagcagatc ttctagagtc ggggcggccg gccgcttcga
961 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
1021 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
1081 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
1141 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
1201 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
1261 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
1321 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
1381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
1441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
1501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
1561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
1621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
1681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
1741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
1801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
1861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
1921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
1981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
2041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
2101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
2161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
2221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat
2281 gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg
2341 cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga
2401 gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc
2461 cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac
2521 tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc
2581 agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc
2641 gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc
2701 catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt
2761 ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc
2821 atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg
2881 tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag
2941 cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat
3001 cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc
3061 atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa
3121 gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta
3181 ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt
3241 acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
3301 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
3361 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
